BAM index file yog dab tsi?
BAM index file yog dab tsi?

Video: BAM index file yog dab tsi?

Video: BAM index file yog dab tsi?
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Anonim

A BAM file (. bam ua ) yog binary version ntawm SAM ntaub ntawv . A SAM ntaub ntawv (. sam) yog cov ntawv tab-delimited ntaub ntawv uas muaj cov ntaub ntawv sib xyaw ua ke. Indexing : IGV xav kom SAM thiab BAM files tau txheeb los ntawm txoj hauj lwm thiab ua indexed , thiab hais tias cov ntaub ntawv index ua raws li ib qho kev pom zoo rau npe.

Ib yam li ntawd, tib neeg nug, Kuv yuav qhib BAM cov ntaub ntawv li cas?

Los ntawm Cov ntaub ntawv xaiv zaub mov Qhib thiab xaiv BAM cov ntaub ntawv ntawm sab laug. Xaiv khawm ntawm sab xis uas hais tias Ntxiv a BAM file . Coj mus rhaub rau BAM Kuaj Cov ntaub ntawv folder koj downloaded xaiv with_index_with_graph. Nyem Qhib , xaiv mapt.

Qhov thib ob, kuv yuav hloov cov ntaub ntawv BAM rau Fastq li cas? Hauv qab no yog cov kauj ruam los Hloov BAM Cov Ntaub Ntawv rau FASTQ hom. Qhov no yuav ua haujlwm ntawm Linux thiab yuav xav tau SAMTOOLS thiab BEDTOOLS

  1. Download SAMTOOLS.
  2. Extract siv Command tar xzvf samtools-version.tar.bz2.
  3. Nws yuav tsim ib daim nplaub tshev los ntawm lub npe samtools-version.
  4. Txav mus rau cov npe cd samtools-version.

Ib yam li ntawd, kuv yuav hloov cov ntaub ntawv BAM mus rau SAM li cas?

Rau convert SAM rau BAM , peb siv samtools saib hais kom ua. Peb yuav tsum hais tias peb cov tswv yim yog nyob rau hauv SAM format (los ntawm lub neej ntawd nws xav tau BAM ) siv qhov kev xaiv -S. Peb kuj yuav tsum tau hais tias peb xav kom cov zis BAM (los ntawm lub neej ntawd nws tsim BAM ) nrog qhov kev xaiv -b.

Samtools saib ua li cas?

Samtools yog ib txheej ntawm kev siv hluav taws xob uas tswj kev sib tw hauv BAM hom. Nws imports los ntawm thiab xa tawm mus rau SAM (Sequence Alignment / Map), ua sorting, merging thiab indexing, thiab tso cai rau retrieve nyeem nyob rau hauv tej cheeb tsam sai sai. Samtools yog tsim los ua haujlwm ntawm cov kwj deg.

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